duoset elisa  (R&D Systems)

Journal: bioRxiv

Article Title: Female-enriched Eggerthella lenta drives neuroinflammation and IFN-γ via host receptor TLR2

doi: 10.64898/2026.03.16.711194

Figure Lengend Snippet: (A) Pathway enrichment analysis of published RNA-seq data from ileal CD4 + T cells (n = 4 mice/group). DEGs were identified via DESeq2 (|FC| > 1.5, Wald test p < 0.05) and analyzed for cellular proces terms; p values are Fisher’s exact tests (FDR shown; see also Table S10). (B and C) Relative expression (variance stabilized transformation [VST] values) of differentially expressed genes (DEGs) from the Th1 and Th2 cell differentiation pathway displayed by (B) sample and (C) group average. Core Th1 genes are highlighted in red. (D-G) Flow cytometry analysis of the (D, E) ileal and (F, G) colonic lamina propria from monocolonized and GF mice (n = 5-9 mice/group). (D, F) Representative histograms and (E, G) gMFI quantification of IFN-γ. p values are Wilcoxon rank sum tests. Mean + SEM is displayed; each point represents an individual mouse. *p < 0.05. See also Figures S6 and S7 and Tables S9 and S10.

Article Snippet: Supernatants were added to a capture-antibody-treated, BSA-blocked Costar® high-binding plate, prepared according to the R&D Systems Mouse IFN-γ DuoSet ELISA Kit protocol.

Techniques: RNA Sequencing, Expressing, Transformation Assay, Cell Differentiation, Flow Cytometry

Journal: bioRxiv

Article Title: Female-enriched Eggerthella lenta drives neuroinflammation and IFN-γ via host receptor TLR2

doi: 10.64898/2026.03.16.711194

Figure Lengend Snippet: (A-C) IFN-γ quantification by ELISA from helper T cells cultured with heat-killed bacteria under Th1-skewing conditions. (A) E. lenta significantly increases IFN-γ in wt mice (n = 12 replicates/group from 2 experiments). (B) E. lenta strains induce stronger responses than other Actinomycetota (n = 7-8 replicates/strain). (C) Induction is significantly reduced in tlr2 −/− T cells (n = 4-16 replicates/group). p values are Wilcoxon rank sum tests (with FDR correction for B, C). (D) Endpoint fecal levels of E. lenta a measured by plating on selective media (n=6-11 replicates/group). (E-H) EAE phenotypes in wt and tlr2 −/− mice gavaged with media or E. lenta (n = 10-11 mice/group). (E) Incidence, (F) disease score, (G) maximum severity, and (H) peak score proportions. p values are likelihood ratio tests (E), mixed-effects models (F), ANOVA of aligned rank transform [ART] models with Wilcoxon pairwise comparisons (G), or ordinal logistic regression and Fisher’s exact tests (G). Values are mean + SEM (A, C, G), mean (B), percent (E, H), or mean ± SEM (F). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. See also Figures S8-S11.

Article Snippet: Supernatants were added to a capture-antibody-treated, BSA-blocked Costar® high-binding plate, prepared according to the R&D Systems Mouse IFN-γ DuoSet ELISA Kit protocol.

Techniques: Enzyme-linked Immunosorbent Assay, Cell Culture, Bacteria